Determination of Vitamins B and C in the Biomass Grown on the Olive Mill Wastewater in Syria by HPCL Method
Determination of Vitamins B and C in the Biomass Grown on the Olive Mill Wastewater in Syria by HPCL Method

Summary/Abstract: The study includes the results of the pH effect of the extraction solution on water-soluble vitamins in the biomass that grown on the olive mill wastewater (resulting from Syrian olive presses) diluted by urea solutions (4%) 75:25 (OMW; Urea 4%) and grafted with Geoterichum hypha. The vitamins determined by high performance liquid chromatography (HPLC) after extracted it in different pH solutions; Phosphate solution pH = 7, Acetic acid solution 2% pH =2.6 and Sodium hydroxide solution 0.1N pH = 12. The results showed that B1, B3, B6, B7, B9 and C were found in the biomass in different percent amount, according to the extraction solution, but no vitamin B2 was present. .In the pH = 7 the percentage of vitamins in biomass was B6 = 0.006%, B7 = 0.11% and B9 =0.01%. In the acidic medium at pH = 2.6, the percentages of vitamins were B3 = 0.005%, B6 = 0.03%, B7 = 0.45%, B9 = 0.003% and C = 0.025%. In the alkali medium the percentages were as follows: B1 = 0.28%, B7 = 0.03% and B9 = 0.04%.

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