Методы планирования экспериментов при оптимизации питательной среды для стрептомицета
Methods of experiments planning by optimization of the nutrient medium for streptomycete
Author(s): I. V. Zhernosekova, N. P. Chernogor, А. А. Tymchuk, A. I. VinnikovSubject(s): Sociobiology
Published by: Дніпропетровський національний університет імені Олеся Гончара
Keywords: streptomycete; staphylolytic enzymes;
Summary/Abstract: Nutritious medium for the streptomycete have been optimized by a method of experiment planning. Optimization of the fermentative medium by method combined planning of experiment (TFE 24 complete factorial) with a method of concentration gradient motion. Activity of staphylolytic enzymes synthesized by Streptomyces recifensis var. lyticus 2Р-15 was studied as a parameter of the medium optimization. The enzymes activity averaged 5589.4 Un/ml in the medium optimized by a path-of-steepest-ascent method. That exceeds 69– 86 % up on the enzymes activity in a control medium derived by a simplex method of experiment planning.
Journal: Biosystems Diversity
- Issue Year: 18/2010
- Issue No: 1
- Page Range: 20-28
- Page Count: 9
- Language: Russian