Optymalizacja hodowli in vitro Kalanchoe pinnata i Calendula officinalis jako źródło bioaktywnych surowców roślinnych dla przemysłu kosmetycznego i farmaceutycznego: od kultury tkankowej do produktu komercyjnego
Optimization of in vitro culture of Kalanchoe pinnata and Calendula officinalis as a source of bioactive plant raw materials for the cosmetics and pharmaceutical industries: from tissue culture to commercial product

Summary/Abstract: The article presents the initial stages of the in vitro culture process of Kalanchoe pinnata and Calendula officinalis, starting from the acquisition of plant material, through the optimization of culture conditions in bioreactor systems, to the extraction of bioactive compounds used in innovative cosmetic and medicinal products. Particular emphasis was placed on process efficiency, biotechnological improvements and the adaptability of biomass to different industrial sectors. In addition, patent aspects and the potential for commercialization of the technology in the context of the global market of natural cosmetics and pharmaceutical preparations will be discussed.As part of the commissioned research service, comprehensive industrial studies were carried out on selected plant cultures, including the initiation of in vitro cultures for five species: Kalanchoe pinnata, Viola tricolor, Lavandula officinalis, Trifolium pratense and Calendula officinalis. The initiation of in vitro culture has shown that only two species - K. pinnata and C. officinalis - are suitable for large-scale production. The aim of the study was to obtain cultures with different degrees of morphogenesis, including callus and shoot cultures, and then to optimize the breeding conditions for Kalanchoe pinnata, Calendula officinalis.

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