CORRELATION OF NIACIN, NAD AND NADP CONCENTRATION IN BLOOD ASSOCIATED WITH LIPOLYSIS IN PERIPARTAL DAIRY COWS
CORRELATION OF NIACIN, NAD AND NADP CONCENTRATION IN BLOOD ASSOCIATED WITH LIPOLYSIS IN PERIPARTAL DAIRY COWS

Summary/Abstract: The aim of this study was to determine correlation between niacin application and concentration of NAD and NADP in blood of cow in peripartal period, also their relation with NEFA concentration was examined. Experiment included 30 cows (15 experimental and 15 animals in control group). Application of niacin was conducted 2 weeks before and after calving. Nicotinic acid was applicated per os with food, 120 grams per day per cow. Nicotinic acid was in rumen-unprotected form. The control group was not treated with nicotinic acid. Blood samples were taken before the application of niacin, then on the day of calving and last sampling was done first and second week after calving. Parameters such as NAD, NADP were determined. NAD and NADP were determined using colorimetric ELISA method from erythrocytes lysates. Relation between NAD, NADP and NAD/NADP thru experimental weeks was analyzed by Pearson's correlation coefficient and simple linear regression model. Correlation and regression between NAD, NADP and NAD/NADP with NEFA was calculated by same method. Results have showed that cows that had niacin application have higher concentrations of NAD and NADP in blood 1 and 2 week after calving, NEFA was statistically lower in niacin group compared to the control group in all weeks. Correlation between NAD and NADP was positive in niacin group and negative in control group but significant correlation wasn’t founded between NAD and NADP and NEFA in control and niacin group.

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